pcmv creb1 Search Results


pcmv6 creb1 rc210577 plasmids  (OriGene)
91
OriGene pcmv6 creb1 rc210577 plasmids
Pcmv6 Creb1 Rc210577 Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/CREB1+(NM_004379)+Human+Tagged+ORF+Clone/pmc04496213-214-6-12
Average 91 stars, based on 1 article reviews
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pcmv-creb  (Becton Dickinson)
90
Becton Dickinson pcmv-creb
Long-term potentiation of exocytosis and membrane resealing are dependent on <t>CREB,</t> but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with <t>pCMV-CREB133</t> and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).
Pcmv Creb, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/pcmv+creb/pmc00140230-43-0-10
Average 90 stars, based on 1 article reviews
pcmv-creb - by Bioz Stars, 2026-10
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pcmv creb1  (Addgene inc)
92
Addgene inc pcmv creb1
Long-term potentiation of exocytosis and membrane resealing are dependent on <t>CREB,</t> but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with <t>pCMV-CREB133</t> and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).
Pcmv Creb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/Creb1+(Plasmid+%23154942)/pmc10171860-229-71-78
Average 92 stars, based on 1 article reviews
pcmv creb1 - by Bioz Stars, 2026-10
92/100 stars
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pcmv.creb1 plasmid  (Promega)
90
Promega pcmv.creb1 plasmid
Long-term potentiation of exocytosis and membrane resealing are dependent on <t>CREB,</t> but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with <t>pCMV-CREB133</t> and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).
Pcmv.Creb1 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/pmir+creb1+wild+type+plasmid/pmc03672802-33-1-6
Average 90 stars, based on 1 article reviews
pcmv.creb1 plasmid - by Bioz Stars, 2026-10
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pcmv-myc-creb plasmid  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare pcmv-myc-creb plasmid
Long-term potentiation of exocytosis and membrane resealing are dependent on <t>CREB,</t> but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with <t>pCMV-CREB133</t> and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).
Pcmv Myc Creb Plasmid, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/pcmv+myc+creb+plasmid/10__1523_slash_jneurosci__3938___09__2009-60-1-10
Average 90 stars, based on 1 article reviews
pcmv-myc-creb plasmid - by Bioz Stars, 2026-10
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pcmv creb  (OriGene)
90
OriGene pcmv creb
Long-term potentiation of exocytosis and membrane resealing are dependent on <t>CREB,</t> but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with <t>pCMV-CREB133</t> and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).
Pcmv Creb, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+creb1/CREB1+(NM_004379)+Human+Tagged+ORF+Clone+Lentiviral+Particle/pmc08714918-249-87-91
Average 90 stars, based on 1 article reviews
pcmv creb - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Long-term potentiation of exocytosis and membrane resealing are dependent on CREB, but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with pCMV-CREB133 and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).

Journal:

Article Title: Long-Term Potentiation of Exocytosis and Cell Membrane Repair in Fibroblasts

doi: 10.1091/mbc.E02-01-0056

Figure Lengend Snippet: Long-term potentiation of exocytosis and membrane resealing are dependent on CREB, but independent of PKA activation. (A) FM1-43 destaining at initial and second wounds in CREB133 cells. 3T3 cells were transfected with pCMV-CREB133 and selected at 600 μg/ml G418. These cells were initially wounded by injection of fura-dextran as a marker, and loaded with 4 μM FM1-43 overnight. Each dish was washed with 1.8 mM Ca2+ Ringer's just before the experiment. The destaining was compared with those of cells that had not been previously wounded and marked by the injection of dextran. CREB133 destaining at a second wound was significantly different from controls. Values are mean ± SE, p = 0.0075. (B) Resealing rate of initial and second wounds in KCREB and CREB133 cells. 3T3 cells were transfected either with pCMV-KCREB or pCMV-CREB133, and stable transfectants were selected at 600 μg/ml G418. The cells were initially wounded by injection of fluorescein-dextran, and resealing rates of second wounds were compared with those of cells that had not been previously wounded. Resealing rates ± SE. Potentiation is still significant at 3 h (p = 0.0133 [control vs. 3 h] in CREB133 cells). (C) Inhibition of PKA during initial wounding does not affect long-term potentiation of membrane resealing. Cells were initially wounded by injection of fluorescein-dextran in the presence of 1 μM CMIQ and washed 15 min after the injection. Resealing rates were compared between noninjected and injected cells 24 h later (p = 0.0236 [control vs. 3 h] and p = 0.0113 [control vs. 24 h]). (D) Forskolin treatment does not induce long-term potentiation of membrane resealing. Cells were treated with 50 μM forskolin for 15 min and wounded 24 h later. Resealing rates were compared with those of nontreated cells. Resealing was not significantly different from controls (p = 0.2071).

Article Snippet: Stable Transfection CREB vectors pCMV-CREB, pCMV-KCREB, and pCMV-CREB133 were from BD Biosciences Clontech (Palo Alto, CA).

Techniques: Activation Assay, Transfection, Injection, Marker, Inhibition